Introducing a Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas

Research demonstrates the feasibility of using type I CRISPR-Cas to effectively introduce a spectrum of long-range chromosomal deletions with a single RNA guide in human embryonic stem cells and HAP1 cells. Type I CRISPR systems rely on the multi-subunit ribonucleoprotein (RNP) complex Cascade to identify DNA targets and on the helicase-nuclease enzyme Cas3 to degrade DNA processively.

CRISPR offshoot still makes mistakes editing DNA, raising concerns about its medical use

Variations of the genome editor CRISPR have wowed biology labs around the world over the past few years because they can precisely change single DNA bases. But such “base editors” can have a serious weakness. A pair of studies published this week shows that one kind of base editor causes many unwanted—and potentially dangerous— “off-target” genetic changes.