Introducing a Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas

Research demonstrates the feasibility of using type I CRISPR-Cas to effectively introduce a spectrum of long-range chromosomal deletions with a single RNA guide in human embryonic stem cells and HAP1 cells. Type I CRISPR systems rely on the multi-subunit ribonucleoprotein (RNP) complex Cascade to identify DNA targets and on the helicase-nuclease enzyme Cas3 to degrade DNA processively.

Corn and other important crops can now be gene edited by pollen carrying CRISPR

CRISPR has transformed many areas of biology but using this tool to enhance certain varieties of crops such as wheat and corn remains difficult because of the plants’ tough cell walls. Now, an agricultural company has solved that problem by using pollen from one genetically modified plant to carry CRISPR’s components into another plant’s cells.

God in a kit: the perils and possibilities of a tool called CRISPR

Alex Kelly, BioFoundry’s 26-year-old lab manager, compares biotech to the birth of the computer, once cloistered in big corporations, now at everyone’s fingertips. Upskill the people in biotech, Kelly believes, and you’ll get disruption writ large, with a potentially massive payoff for the most disadvantaged.

CRISPR offshoot still makes mistakes editing DNA, raising concerns about its medical use

Variations of the genome editor CRISPR have wowed biology labs around the world over the past few years because they can precisely change single DNA bases. But such “base editors” can have a serious weakness. A pair of studies published this week shows that one kind of base editor causes many unwanted—and potentially dangerous— “off-target” genetic changes.